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21:23
YouTube
Andrew Ng
POV: How To Do a Transfection
This video illustrates how to transfect mammalian cells with plasmid DNA using lipid based reagents. In this experiment I used Fugene HD to transfect LxHEK293T cells (https://www.takarabio.com/products/gene-function/viral-transduction/lentivirus/packaging-systems-and-cells/lenti-x-293t-cells) with a mixture of three plasmids to produce ...
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